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SKU Number : 414154

GENE-UP® STEC – Top 6 (EH2) is a real-time Polymerase Chain Reaction (PCR) assay for the detection of non-O157 EHEC serotypes in food products This test is intended for use only as part of the EHEC detection method for the screening of EHEC.

The GENE-UP® STEC – Top 6 kit contains components for 24 reactions total.All required strip tubes and caps are included in the kit; additional strips and tubes must be purchased separately.

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The GENE-UP® STEC – Top 6 kit is to be used with compatible PCR strip tubes in the GENE-UP® Thermocycler. Each reaction vial in the GENE-UP® STEC – Top 6 kit contains all of the necessary components for PCR, including sample-specific primers and probes and an internal amplification control.

All the assays for the sample and the internal amplification control utilize Molecular Beacon hybridization probes. These probes consist of one short oligonucleotides that hybridize to an internal sequence of the amplified fragment during the annealing phase of the reaction cycle. The signal occurs only after the probes come in close proximity from hybridizing to the template DNA.

The resulting fluorescent signal, which forms a real-time amplification curve, is how the amplified target is detected by the GENE-UP® Thermocycler. The GENE-UP STEC – Top 6 kit allows the simultaneously detection of E. coli O26, O45, O103, O111, O121, and O145.